Select the one best response to each question!
Which technique is based on probe amplification rather than amplification of the target in question?
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How are DNA and RNA different?
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All of the following are true of a nucleic acid probe except
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Which of the following techniques uses restriction enzymes, electrophoresis, and then transfer of DNA fragments onto a solid matrix, followed by probing with labeled probes?
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All of the following would be advantages of nucleic acid amplification techniques except
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Which best describes the principle of DNA chip technology?
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A hybridization reaction involves which of the following?
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Wrong!
Which best describes the PCR?
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During PCR, what happens in the annealing step?
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Wrong!
What is the function of restriction endonucleases?
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Wrong!
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To what does in situ hybridization refer?
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What is PCR used for in clinical settings?
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[Immunology] Molecular Diagnostic Techniques Quizzes (12 tests)
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| 1. All of the following may contribute to autoimmunity except a. clonal deletion of self-reactive T cells. b. molecular mimicry. c. new expression of class II MHC antigens. d. polyclonal activation of B cells. |
| 2. Which of the following would be considered an organspecific autoimmune disease? a. SLE b. RA c. Hashimoto’s thyroiditis d. Goodpasture’s syndrome |
| 3. SLE can be distinguished from RA on the basis of which of the following? a. Joint pain b. Presence of antinuclear antibodies c. Immune complex formation with activation of complement d. Presence of anti-ds DNA antibodies |
| 4. Which of the following would support a diagnosis of drug-induced lupus? a. Antihistone antibodies b. Antibodies to Smith antigen c. Presence of RF d. Antibodies to SS-A and SS-B antigens |
| 5. A homogeneous pattern of staining of the nucleus on IIF may be caused by which of the following antibodies? a. Anti-Sm antibody b. Anti-SSA/Ro antibody c. Antihistone antibody d. Anti–double-stranded DNA |
| 6. Which of the following is characteristic of RA? a. Association with certain HLA-DR genes b. Joint involvement that is symmetric c. Presence of antibody against IgG d. All of the above |
| 7. Which of the following best describes the slide agglutination test for RF? a. It is specific for RA. b. A negative test rules out the possibility of RA. c. It is a sensitive screening tool. d. It detects IgG made against IgM |
| 8. Hashimoto’s thyroiditis can best be differentiated from Graves’ disease on the basis of which of the following? a. Decrease in thyroid hormone levels b. Presence of thyroid peroxidase antibodies c. Enlargement of the thyroid d. Presence of lymphocytes in the thyroid |
| 9. Which of the following would be considered a significant finding in Graves’ disease? a. Increased TSH levels b. Antibody to TSHR c. Decreased T3 and T4 d. Antithyroglobulin antibody |
| 10. Immunologic findings in type I diabetes mellitus include all of the following except a. presence of CD8 T cells in the islets of Langerhans. b. antibody to colloid. c. antibody to insulin. d. antibody to GAD. |
| 11. Destruction of the myelin sheath of axons caused by the presence of antibody is characteristic of which disease? a. MS b. MG c. Graves’ disease d. Goodpasture’s syndrome |
| 12. Blood was drawn from a 25-year-old woman with suspected SLE. A FANA screen was performed, and a speckled pattern resulted. Which of the following actions should be taken next? a. Report out as diagnostic for SLE b. Report out as drug-induced lupus c. Perform an antibody profile d. Repeat the test |
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