[Immunology] Precipitation Reactions Quizzes (14 tests)

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[Immunology] Precipitation Reactions Quizzes (14 tests)
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Select the one best response to each question!

In a precipitation reaction, how can the ideal antibody be characterized?


1. In a precipitation reaction, how can the ideal antibody be characterized?
a. Low affinity and low avidity
b. High affinity and low avidity
c. High affinity and high avidity
d. Low affinity and high avidity
2. Precipitation differs from agglutination in which way?
a. Precipitation can only be measured by an automated instrument.
b. Precipitation occurs with univalent antigen, while agglutination requires multivalent antigen.
c. Precipitation does not readily occur, because few antibodies can form aggregates with antigen.
d. Precipitation involves a soluble antigen, while agglutination involves a particulate antigen.
3. When soluble antigens diffuse in a gel that contains antibody, in which zone does optimum precipitation occur?
a. Prozone
b. Zone of equivalence
c. Postzone
d. Prezone
4. Which of the following statements apply to rate nephelometry?
a. Readings are taken before equivalence is reached.
b. It is more sensitive than turbidity.
c. Measurements are time dependent.
d. All of the above
5. Which of the following is characteristic of the end-point method of RID?
a. Readings are taken before equivalence.
b. Concentration is directly in proportion to the square of the diameter.
c. The diameter is plotted against the log of the concentration.
d. It is primarily a qualitative rather than a quantitative method.
6. Which statement is true of measurements of turbidity?
a. It indicates the ratio of incident light to transmitted light.
b. Light that is scattered at an angle is detected.
c. It is recorded in units of relative light scatter.
d. It is not affected by large particles falling out of solution.
7. Which of the following refers to the force of attraction between an antibody and a single antigenic determinant?
a. Affinity
b. Avidity
c. Van der Waals attraction
d. Covalence
8. Which technique is typified by radial immunodiffusion combined with electrophoresis?
a. Countercurrent electrophoresis
b. Rocket electrophoresis
c. Immunoelectrophoresis
d. Southern blotting
9. Immunofixation electrophoresis differs from immunoelectrophoresis in which way?
a. Electrophoresis takes place after diffusion has occurred.
b. Better separation of proteins with the same electrophoretic mobilities is obtained.
c. Antibody is directly applied to the gel instead of being placed in a trough.
d. It is mainly used for antigen detection.
10. In which zone might an antibody-screening test be false negative?
a. Prozone
b. Zone of equivalence
c. Postzone
d. None of the above
11. If crossed lines result in an Ouchterlony immunodiffusion reaction with antigens 1 and 2, what does this indicate?
a. Antigens 1 and 2 are identical.
b. Antigen 2 is simpler than antigen 1.
c. Antigen 2 is more complex than antigen 1.
d. The two antigens are unrelated.
12. Which might affect the outcome of immunodiffusion procedures?
a. Improper dilution of antigen in the wells
b. Overfilling the wells
c. Nonlevel incubation of plates
d. All of the above
13. Which technique represents a single-diffusion reaction?
a. Radial immunodiffusion
b. Ouchterlony diffusion
c. Immunoelectrophoresis
d. All of the above
14. Which best describes the law of mass action?
a. Once antigen–antibody binding takes place, it is irreversible.
b. The equilibrium constant depends only on the forward reaction.
c. The equilibrium constant is related to strength of antigen–antibody binding.
d. If an antibody has a high avidity, it will dissociate from antigen easily.

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