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Select the one best response to each question!
Which technique is based on probe amplification rather than amplification of the target in question?
| 1. Which technique is based on probe amplification rather than amplification of the target in question? a. PCR b. TMA c. Dot-blot d. Branched chain amplification |
| 2. How are DNA and RNA different? a. Only RNA contains uracil. b. Only DNA contains cytosine. c. DNA is less stable than RNA. d. RNA is usually double-stranded. |
| 3. All of the following are true of a nucleic acid probe except a. it is a short nucleic acid chain. b. it can be made up of either DNA or RNA. c. it is labeled with a marker for detection. d. it attaches to double-stranded DNA. |
| 4. Which of the following techniques uses restriction enzymes, electrophoresis, and then transfer of DNA fragments onto a solid matrix, followed by probing with labeled probes? a. Dot-blot b. Southern blot c. Hybridization protection assay d. LCR |
| 5. All of the following would be advantages of nucleic acid amplification techniques except a. detection of nonviable organisms. b. extreme sensitivity. c. early detection of disease. d. low cost. |
| 6. Which best describes the principle of DNA chip technology? a. Chips contain multiple probes on their surface. b. Chips contain multiple copies of one unique probe. c. The sample is labeled with a fluorescent tag. d. Hybridization is detected by the presence of radioactivity |
| 7. A hybridization reaction involves which of the following? a. Binding of two complementary DNA strands b. Cleaving of DNA into smaller segments c. Separating DNA strands by heating d. Increasing the number of DNA copies |
| 8. Which best describes the PCR? a. Two probes are joined by a ligating enzyme. b. RNA copies of the original DNA are made. c. Extender probes are used to detect a positive reaction. d. Primers are used to make multiple DNA copies. |
| 9. During PCR, what happens in the annealing step? a. The primers bind to the target DNA. b. Strands are separated by heating. c. An RNA copy is made. d. Protein is made from the DNA strands. |
| 10. What is the function of restriction endonucleases? a. They splice short DNA pieces together. b. They cleave DNA at specific sites. c. They make RNA copies of DNA. d. They make DNA copies from RNA. |
| 11. To what does in situ hybridization refer? a. Nucleic acid probes react with intact cells within tissues. b. Probes are protected from degradation if hybridized. c. RNA polymerase copies messenger RNA. d. Hybridization takes place in solution. |
| 12. What is PCR used for in clinical settings? a. Detection of early HIV infection b. Determination of specific HLA antigens c. Measurement of cytokines d. All of the above |
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