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Select the one best response to each question!
Flow cytometry separates cells on the basis of which of the following?
|1. Flow cytometry separates cells on the basis of which of the following?
a. Forward and 90-degree side scatter of an interrupted beam of light
b. Front-angle scatter only of an interrupted light beam
c. Absorbance of light by different types of cells
d. Transmittance of light by cells in solution
|2. Forward-angle light scatter is an indicator of cell
|3. What is the single most important requirement for samples to be analyzed on a flow cytometer?
a. Whole blood is collected into a serum-separator tube.
b. Cells must be in a single-cell suspension.
c. Samples must be fixed in formaldehyde prior to processing.
d. Blood must be kept refrigerated while processing.
|4. Which represents the best explanation for a flow cytometer’s ability to detect several cell surface markers at the same time?
a. The forward scatter can separate out cells on the basis of complexity.
b. One detector can be used to detect many different wavelengths.
c. For each marker, a specific fluorochrome–antibody combination is used.
d. Intrinsic parameters are separated out on the basis
of amount of side scatter.
|5. Which of the following cell surface markers would be present on a population of T helper cells?
a. CD3 and CD4
b. CD3 and CD8
c. CD3 only
d. CD4 only
|6. Which cell surface marker is termed the common acute lymphoblastic leukemia marker?
|7. All of the following are clinical applications for flow cytometry except
a. fetal hemoglobin.
b. immunophenotyping of lymphocyte subpopulations.
c. HIV viral load analysis.
d. enumeration of stem cells in a peripheral blood mononuclear cell product.
|8. Which type of analyzer allows one to measure multiple analytes from multiple samples, loaded at any time?
a. Batch analyzer
b. Random access analyzers
c. Front-end loaded analyzers
d. Sequential access analyzers
|9. All of the following are benefits of automation except
a. greater accuracy.
b. increased turnaround time.
c. savings on controls.
d. less disposal of outdated reagents.
|10. If an analyzer gets different results each time the same sample is tested, what type of problem does this represent?