[MCQs] The quizzes about Material Employed in Hematology (50 test)

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1. When using laboratory reagents, what routine hematological care should be taken in their preparation?

1. Follow detailed procedural directions.
2. Measure regents exactly.
3. Follow the order that reagents are to be added.
4. Control the temperature where indicated.
5. Use filtration of ingredients as stated.
6. Age the prepared solution as specified.
7. All of the above.

2. Particular attention must be given to storage of laboratory reagents, particularly with reference to requirements for:

1. Refrigeration.
2. Incubation.
3. Protection from intense light.
4. All of the above.

3. A reagent container is correctly labeled if the label contains the:

1. Constituents, initials of the individual who prepared the reagent in alphabetical order, expiration date, quantity used, and date
2. Expiration date, initials of the individual who prepared the reagent, constituents quantity used, temperature control, and date
3. Initials of the individual who prepared the reagent, stains, constituents, expiration date, quantity used, and date
4. Constituents, expiration date, initials of the individual who prepared the reagent, quantity used, and date prepared.

4. A reagent’s container label is properly labeled and protected if the label is:

1. Complete and securely attached.
2. Neatly and legibly written or preferably
3. Covered with a protective coating of cellophane tape over the surface of the label.
4. All of the

5. Why must an unused portion of a reagent never be poured back into tile original container?

1. Possible explosions.
2. Inefficiency.
3. Contamination.
4. Improper labeling

6. When storing reagents on shelving, protect them from:

1. Dust.
2. Moisture.
3. Direct sunlight.
4. All of the above.

7. What should you do if you cannot clearly read the reagent’s label and/or identify the contents of the container?

1. Use the
2. Check with your
3. Discard it properly and use a reagent’s container with a label that you can read and the contents you can
4. Save it for next

8. When preparing laboratory reagents for storage, which items should be chemically cleaned prior to use?

1. Mixing
2. Stirring rods.
3. Storage
4. All of the

9. At what point is it is a good, safe, practice to avoid contact of reagents with metals since metals may become unusable for laboratory work?

1. Labeling and
2. Refrigeration and
3. Preparation and
4. Preparation and

10. Highly toxic reagents should be conspicuously labeled:

1. Reagent.
2. “POISON.”
3. Poison.
4. “REAGENT”.

11. The white blood cell diluting pipet has a:

1. Smaller bulb, a red bead, and an “11.”
2. Smaller bulb, a white bead, and an “11.”
3. Larger bulb, a red bead, and a “101.”
4. Larger bulb, a white bead, and a “101.”

12. The red blood cell diluting pipet has a:

1. Smaller bulb, a red bead, and an “11.”
2. Smaller bulb, a white bead, and an “11.”
3. Larger bulb, a red read, and a “101.”
4. Larger bulb, a white read, and a “101.”

13. The 0.5 mark on the RBC diluting pipet designates a volume equal to:

1. 5 ml.
2. 5 cu mm.
3. 1/20 of the dilution
4. 1/200 of the dilution

14. Which statement is correct concerning the use of the diluting pipets for cell counting?

1. Blood or fluid is drawn into the pipet to a predetermined point and diluted to the correct mark with diluting fluid. After proper mixing, the diluted substance is placed in the counting chamber and the cells are counted.
2. Blood or fluid is drawn into the pipet to an arbitrary point and diluted to the correct mark with diluting fluid. After proper mixing, the diluted substance is placed in the counting chamber and the cells are counted.
3. Blood or fluid is drawn into the pipet to a predetermined point and diluted to a guesstimated mark with diluting fluid. After gentle mixing, the diluted substance is placed in the counting chamber and the cells are counted.

15. Usually, the technique for diluting the blood specimen with a pipet calls for whole blood to be diluted only to the “11” or “101” mark with appropriate diluting fluid dependent upon the type of cell count. How is the dilution calculated?

1. The volume of fluid is measured in the stem and then
2. Dilution is calculated on the volume in the
3. Both a and
4. None of the above

16. In general, what is the proper procedure for puncturing the diaphragm using the Unopette System?

1. Using the protective shield on the capillary pipette, puncture the diaphragm of the reservoir.
2. Grasping the reservoir in one hand, take pipette assembly in other hand and pull tip of pipette shield firmly through diaphragm in neck of reservoir, then remove.
3. Grasping the reservoir in one hand, take pipette assembly in other hand and push tip of pipette shield firmly through diaphragm in neck of reservoir, then remove.
4. Remove shield from pipette assembly with a twist.
5. a, b, c, and
6. a, b, and
7. a, c, and

17. What phenomenon is used to fill the Unopette capillary with blood?

1. Gravity.
2. Capillary action.
3. Brownian movement.
4. Static electricity.

18. When using the Unopette system, at what point will the pipette automatically stop filling and be complete? When:

1. The blood reaches the top
2. Negative pressure is applied,
3. The blood reaches the end of the capillary

19. How many times should the reservoir be squeezed and with what pressure to raise the capillary bore and force the diluent up into, but not out of, the overflow chamber using the Unopette system?

1. 4 to 6;
2. 3 to 5;
3. 2 to 3;
4. 1 to 6;

20. Using the Unopette System, after the blood is thoroughly mixed with the diluent and left to stand for 10 minutes, the red cells will:

1. Overflow.
2. Settle out.
3. Hemolyze.

21. What are the outer dimensions of the Neubauer ruling?

1. 0.20 by 0.20 mm.
2. 0.25 by 0.25 mm.
3. 1 by 1 mm.
4. 3 by 3 mm.

22. What configuration does the most common type of hemacytometer look like for counting blood cells?

1. Two counting chambers separated by grooves or canals.
2. Three counting chambers separated by grooves or canals.
3. Two counting chambers separated by rough indentations.
4. Four counting chambers that are not separated.

23. How many squares are used to count white cells, when the dimensions of the Neubauer ruling are further divided into 9 smaller squares, with dimensions of 1 mm by 1 mm?

1. 2
2. 3
3. 4
4. 5

24. Using the Neubauer ruling for the red cell count, which portion and how many mms are used?

1. Middle squares; 0 30 mm on a side.
2. Outer 8 squares; 0.15 mm on a side.
3. 10 outer squares; 0 05mm on a side.
4. Central square; 0.20 mm on a side.

25. When taking a blood cell count, the cover glass must be free of visible                                       and optically                                      on both sides.

1. Defects; plane.
2. Outer squares;
3. Stains; rough.
4. Blood; clean.

26. A method for cleaning Wintrobe sedimentation rate tubes, small bore tubes and pipets, and remaining blood clots in the bore of a pipet is to:

1. Attach a capillary pipet by a rubber hose, to a water type suction
2. Attach the tube to the flat end of the pipet and hold the tube under
3. Draw the blood from the tube as water is drawn
4. Ensure the tube is clean by inverting it, remove the residual water by suction, and allow to dry.
5. All of the
6. None of the

27. What occasional method it used to clean small bore tubes and pipets?

1. Use dilute sodium hypochlorite (household bleach) to remove deposits of residual
2. Use distilled
3. Insert a small rubber
4. Use a suction
5. Absorbent lens paper and

28. Blood dilution pipets can be washed by:

1. Soaking in calcium
2. Soaking in ammonia.
3. Flushing water and acetone through
4. Rinsing in distilled

29. Glassware, which is used for coagulation studies, must be                                                cleaned in                                                            .

1. Gently; household
2. Freely; distilled
3. Scrupulously; non-organic
4. Somewhat organic soapy
5. Vigorously; in water with absorbent lens

30. Select the correct items normally found on a modern microscope used in a hematology laboratory.

1. A darkening system, a substage condenser system, an objective system, a projector (eyepiece or ocular system), an iris diaphragm, nicol prisms, a tubular barrel (monocular or binocular bodies), and a mechanical stage.
2. An illuminator system, a substage condenser system, an objective system, a projector (eyepiece or ocular system), an iris diaphragm, nicol prisms, a tubular barrel (monocular or binocular bodies), and a mechanical
3. An illuminator system, a substage condenser system, an objective system, a projector (eyepiece or ocular system), a round barrel (monocular or binocular bodies), and a mechanical stage.
4. An illuminator system, a substage condenser system, an objective system, a projector (eyepiece or ocular system), an iris diaphragm, nicol prisms, a tubular barrel (monocular or binocular bodies), and a mechanical

31. What combination of lenses does a compound microscope use?

1. Objective
2. Monocular
3. Aerial image
4. a and b
5. b and c

32. Select the best explanation of an “aerial image”.

1. An image formed in the
2. An image formed in the air. The object is viewed through the projector or eyepiece that acts like a magnifier except that it magnifies an aerial object instead of an actual
3. An image formed in the air. The object is viewed through the magnifying glass except that it magnifies an aerial object instead of an actual
4. An image formed on a surface. The object is viewed through the projector or eyepiece that acts like a magnifier except that it magnifies an aerial object instead of an actual object.

33. The ability of a microscope to render fine detail is dependent upon the numerical aperture and proper adjustment of which lens (es)?

1. Ocular and
2. Ocular and
3. Objective and
4. Objective

34. When rotation of a microscope’s fine adjustment causes an object in the center of the field to sway from side to side, the lighting is:

1. Central.
2. Oblique.
3. Too dim.
4. Too intense.

35. Name one item the resolving power of the microscope is dependent upon during magnification?

1. Focal
2. Binocular
3. Arial
4. A. of the objective.

36. Preliminary focusing of a microscope should be observed from the:

1. Ocular.
2. Objective.
3. Top of the microscope.
4. Side of the microscope.

37. Since correct illumination of an object under study is an extremely important detail, what can incorrect lighting cause?

1. Inaccurate results and conclusions.
2. Inaccurate steps and timings.
3. Faulty conclusions and recommendations.
4. Changing positions and recommendations.

38. What is the function of the substage condenser when illuminating slides under the microscopic?

1. Indicates dark
2. Reduces glare.
3. Directs a light
4. Correct inconsistencies by changing the position left or

39. Which statement about the substage condenser is true?

1. The substage condenser functions to direct a hair beam of the desired numerical aperture (N.A.) and field size onto the specimen.
2. The size of the opening in the condenser together with its position up or down controls the light entering the system.
3. When the condenser is open to the stage, concentration of light is greater.
4. As the condenser is moved upward, less light passes downward through the object under observation.

40. Improper illumination is indicated when:

1. Light points appear on the outer edges of the slide.
2. The center of an object is bright on one side and dark on the other.
3. The object appears to be in dull light
4. Shadows appear in the field.

41. Which three parts of a microscope may be adjusted to control the illumination?

1. Mirror, iris diaphragm, and ocular.
2. Mirror, iris diaphragm, and objective.
3. Mirror, iris diaphragm, and condenser.
4. Ocular, objective, and condenser.

42. When using a microscope the “virtual image” projected on the retina of the eye is the image.

1. Initial.
2. Intermediate.
3. Final.
4. Aperture.

43. Which of the following is used to clean microscope lenses?

1. Xylene.
2. Acetone.
3. Household bleach.
4. Saturated sodium hydroxide.

44. Which type of microscope is preferred for routine hematology?

1. Monocular.
2. Binocular.
3. Fluorescence.
4. Phase

45. Because of dust, the lens system should:

1. Be separated.
2. Never be separated.

46. After a capillary centrifuge tube is filled with blood, it is sealed with:

1. Plastic sealing material.
2. Paper.
3. Glass.
4. Wax.

47. Most microhematocrit centrifuges have a speed of about:

1. 500 rpm
2. 1,000 rpm.
3. 5, 000 rpm.
4. 10,000 rpm.

48. Which centrifuge has the higher type of top speed for instruments?

1. Table top model.
2. Floor-mounted model.

49. Only which agent should be used to remove oil from a lens object?

1. An abrasive.
2. A rag.
3. Xylene.
4. Camel hairbrush.

50. Whenever centrifugation is required, which precaution must always be followed?

1. Careful attention must be given to balancing the units.  This  means that tubes must be placed exactly opposite each other, they must be of identical weight, and they must contain the same amount of fluid.
2. Centrifuges should be equipped with a microhematocrit so that speed may be checked and controlled.
3. Hematocrits require a regular force and can adapt for 6 to 19  tubes.
4. The heavier, floor-mounted models require rubber feet to absorb vibrations so as to accommodate a large number of tubes, which are housed in mounted receptacles on spokes from a distance hub.