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NUCLEIC ACIDS- Part 15
See all quizzes of NUCLEIC ACIDS- Part 15 here:
1 In sticky ends produced by restriction
endonucleases
(A) The 2 strands of DNA are joined to each other
(B) The DNA strands stick to the restriction
endonuclease
(C) The ends of a double stranded fragment are
overlapping
(D) The ends of a double stranded fragment are
non overlapping
2. All of the following may be used as expression
vectors except
(A) Plasmid (B) Bacteriophage
(C) Baculovirus (D) E. coli
3. A plasmid is a
(A) Single stranded linear DNA
(B) Single stranded circular DNA
(C) Double stranded linear DNA
(D) Double stranded circular DNA
4. Fragments of DNA can be identified by the
technique of
(A) Western blotting (B) Eastern blotting
(C) Northern blotting (D) Southern blotting
5. A particular RNA in a mixture can be
identified by
(A) Western blotting (B) Eastern blotting
(C) Northern blotting (D) Southern blotting
6. A radioactive isotope labeled cDNA probe
is used in
(A) Southern blotting (B) Northern blotting
(C) Both (A) and (B) (D) None of these
7. An antibody probe is used in
(A) Southern blotting (B) Northern blotting
(C) Western blotting (D) None of these
8. A particular protein in a mixture can be
detected by
A) Southern blotting (B) Northern blotting
(C) Western blotting (D) None of these
9. The first protein synthesized by recombinant
DNA technology was
(A) Streptokinase
(B) Human growth hormone
(C) Tissue plasminogen activator
(D) Human insulin
10. For production of eukaryotic protein by
recombinant DNA technology in bacteria,
the template used is
(A) Eukaryotic gene (B) hnRNA
(C) mRNA (D) All of these
11. Monoclonal antibodies are prepared by
cloning
(A) Myeloma cells (B) Hybridoma cells
(C) T-Lymphocytes (D) B-Lymphocytes
12. Myeloma cells are lacking in
(A) TMP synthetase
(B) Formyl transferase
(C) HGPRT
(D) All of these
13. Hybridoma cells are selected by culturing
them in a medium containing
(A) Adenine, guanine, cytosine and thymine
(B) Adenine, guanine, cytosine and uracil
(C) Hypoxanthine, aminopterin and thymine
(D) Hypoxanthine, aminopterin and thymidine
14. Myeloma cells and lymphocytes can be
fused by using
(A) Calcium chloride (B) Ethidium bromide
(C) Polyethylene glycol (D) DNA polymerase
15. Trials for gene therapy in human beings
were first carried out, with considerable
success, in a genetic disease called
(A) Cystic fibrosis
(B) Thalassemia
(C) Adenosine deaminase deficiency
(D) Lesch-Nyhan syndrome
16. Chimeric DNA
(A) Is found in bacteriophages
(B) Contains unrelated genes
(C) Has no restriction sites
(D) Is palindromic
17. Which of the following may be used as a
cloning vector?
(A) Prokaryotic plasmid (B) Lambda phage
(C) Cosmid (D) All of these
18. The plasmid pBR322 has
(A) Ampicillin resistance gene
(B) Tetracycline resistance gene
(C) Both (A) and (B)
(D) None of these
19. Lambda phage can be used to clone DNA
fragments of the size
(A) Upto 3 kilobases (B) Upto 20 kilobases
(C) Upto 45 kilobases (D) Upto 1,000 kilobases
20. DNA fragments upto 45 kilobases in size
can be cloned in
(A) Bacterial plasmids
(B) Lambda phage
(C) Cosmids
(D) Yeast artificial chromosomes
21. A cosmid is a
(A) Large bacterial plasmid
(B) Viral plasmid
(C) Hybrid of plasmid and phage
(D) Yeast plasmid
22. Polymerase chain reaction can rapidly
amplify DNA sequences of the size
(A) Upto 10 kilobases (B) Upto 45 kilobases
(C) Upto 100 kilobases(D) Upto 1,000 kilobases
23. The DNA polymerase commonly used in
polymerase chain reaction is obtained from
(A) E. coli (B) Yeast
(C) T.aquaticus (D) Eukaryotes
24. Base sequence of DNA can be determined
by
(A) Maxam-Gilbert method
(B) Sanger’s dideoxy method
(C) Both (A) and (B)
(D) None of these
25. From a DNA-RNA hybrid, DNA can be
obtained by addition of
(A) DNA B protein and ATP
(B) Helicase and ATP
(C) DNA topoisomerase I
(D) Alkali
26. Optimum temperature of DNA polymerase
of T. aquaticus is
(A) 30°C (B) 37°C
(C) 54°C (D) 72°C
27. In addition to Taq polymerase, polymerase
chain reaction requires all of the
following except
(A) A template DNA
(B) Deoxyribonucleoside triphosphates
(C) Primers
(D) Primase
28. DNA polymerase of T. aquaticus is
preferred to that of E. coli in PCR because
(A) It replicates DNA more efficiently
(B) It doesn’t require primers
(C) It is not denatured at the melting temperature
of DNA
(D) It doesn’t cause errors in replication
29. Twenty cycles of PCR can amplify DNA:
(A) 220 fold (B) 202 fold
(C) 20 x 2 fold (D) 20 fold
30. Transgenic animals may be prepared by
introducing a foreign gene into
(A) Somatic cells of young animals
(B) Testes and ovaries of animals
(C) A viral vector and infecting the animals with
the viral vector
(D) Fertilised egg and implanting the egg into a
foster mother
