[Immunology] Molecular Diagnostic Techniques Quizzes (12 tests)

Which technique is based on probe amplification rather than amplification of the target in question?

PCR

TMA

Dot-blot

Branched chain amplification

Correct! Wrong!

How are DNA and RNA different?

Only RNA contains uracil.

Only DNA contains cytosine.

DNA is less stable than RNA.

RNA is usually double-stranded.

Correct! Wrong!

All of the following are true of a nucleic acid probe except

it is a short nucleic acid chain.

it can be made up of either DNA or RNA.

it is labeled with a marker for detection.

it attaches to double-stranded DNA.

Correct! Wrong!

Which of the following techniques uses restriction enzymes, electrophoresis, and then transfer of DNA fragments onto a solid matrix, followed by probing with labeled probes?

Dot-blot

Southern blot

Hybridization protection assay

LCR

Correct! Wrong!

All of the following would be advantages of nucleic acid amplification techniques except

detection of nonviable organisms.

extreme sensitivity.

early detection of disease.

low cost.

Correct! Wrong!

Which best describes the principle of DNA chip technology?

Chips contain multiple probes on their surface

Chips contain multiple copies of one unique probe.

The sample is labeled with a fluorescent tag.

Hybridization is detected by the presence of radioactivity

Correct! Wrong!

A hybridization reaction involves which of the following?

Binding of two complementary DNA strands

Cleaving of DNA into smaller segments

Separating DNA strands by heating

Increasing the number of DNA copies

Correct! Wrong!

Which best describes the PCR?

Two probes are joined by a ligating enzyme.

RNA copies of the original DNA are made.

Extender probes are used to detect a positive reaction.

Primers are used to make multiple DNA copies.

Correct! Wrong!

During PCR, what happens in the annealing step?

The primers bind to the target DNA.

Strands are separated by heating.

An RNA copy is made.

Protein is made from the DNA strands.

Correct! Wrong!

What is the function of restriction endonucleases?

They splice short DNA pieces together.

They cleave DNA at specific sites.

They make RNA copies of DNA.

They make DNA copies from RNA.

Correct! Wrong!

To what does in situ hybridization refer?

Nucleic acid probes react with intact cells within tissues.

Probes are protected from degradation if hybridized.

RNA polymerase copies messenger RNA.

Hybridization takes place in solution.

Correct! Wrong!

What is PCR used for in clinical settings?

Detection of early HIV infection

Determination of specific HLA antigens

Measurement of cytokines

All of the above

Correct! Wrong!

[Immunology] Molecular Diagnostic Techniques Quizzes (12 tests)

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[Immunology] Molecular Diagnostic Techniques Quizzes (12 tests)

Which technique is based on probe amplification rather than amplification of the target in question?

How are DNA and RNA different?

All of the following are true of a nucleic acid probe except

Which of the following techniques uses restriction enzymes, electrophoresis, and then transfer of DNA fragments onto a solid matrix, followed by probing with labeled probes?

All of the following would be advantages of nucleic acid amplification techniques except

Which best describes the principle of DNA chip technology?

A hybridization reaction involves which of the following?

Which best describes the PCR?

During PCR, what happens in the annealing step?

What is the function of restriction endonucleases?

To what does in situ hybridization refer?

What is PCR used for in clinical settings?

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