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Collection of Blood and Preparation of Blood Smears - Part 2
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1. What is an important vacutainer procedure?
- The short needle should be embedded in the stopper, but the needle must not break the vacuum.
- Any needle should be embedded in the stopper, but the needle must not break the vacuum.
- The long needle should be embedded in the stopper, but the needle must not break the vacuum.
- The first needle should be embedded in the stopper, but the needle must not break the vacuum.
2. If the multiple needle is used or more than one tube is required for venipuncture, what is to be followed?
- Tighten the tourniquet after the first tube is filled; remove the filled tube and insert the next one.
- Loosen the tourniquet after the first tube is filled; remove the filled tube.
- Release the tourniquet after the first tube is filled; remove the filled tube and insert the next one.
- Tighten the tourniquet after the first tube is filled and insert the next one.
3. Why must you be careful not to remove the needle while tubes are being changed?
- The blood will continue to flow.
- The skin may rip.
- a and b may occur separately or at one tine.
4. Why is it most important that correct venipuncture technique be practiced? To:
- Avoid unnecessary pain to the patient.
- Prevent tissue damage.
- Secure a good representative blood specimen.
- Prevent contamination of the specimen or infection of the patient.
- All of the above.
- a, b, and d.
5. What may occur to the donor if the tourniquet is not removed as early as possible once the blood starts flowing well?
- Coagulation of the blood.
- Changes in the quantitative values of the blood components.
- Hemolysis of the blood specimen.
- All of the above.
6. The blood count should be performed within once the blood is
- 30 minutes.
- 3 hours.
- 24 hours.
- 48 hours.
7. The site of a capillary puncture should be:
8. When is the tourniquet released and removed?
- When a hematoma begins to form, the first drop of blood appears, or when it is hard to enter the vein.
- When it is hard to enter the vein, a hematoma begins to form, or the first drop of blood appears, aspiration occurs.
- When a hematoma begins to form, or it is hard to enter the vein.
- All of the above.
9. What will occur if blood is squeezed from a capillary puncture?
- Unnecessary pain.
- Free- flowing punctures.
- Inaccurate test results.
10. Which aspect of the fingertip should be used as the site for a capillarypuncture?
11. Sodium citrate is a good anticoagulant for coagulation studies because:
- A concentration of one part 0.2 M sodium citrate is used to 9 parts of whole blood.
- It binds the calcium of the blood into a soluble complex to prevent coagulation.
- It combines cellular constituents in the plasma.
- A concentration of one part 0.1 M sodium citrate is used to 15 parts of whole blood.
- Does not alter the size of cellular components.
- Dissolves more rapidly than double oxalate salts.
- Is least expensive.
- Can be used in excessive amounts.
13. EDTA ammonium-potassium oxalate, and heparin are commonly used:
14. What are the two basic methods for the preparation of blood smears?
- Cover slip and polychromic stains.
- Slide and acid dye.
- Slide and cover slip.
- Methylene blue and slide.
15. How many solutions are needed to perform a Wright’s stain buffer?
16. What should be the final pH of the buffer used with Wright’s stain? 6.0.
17. Where on a dried blood smear is the name or identification of the patient written?
- Thin area.
- Thick area.
18. The Wright’s stain should remain on a blood smear …………………………………… before the buffer solution is added and should not be allowed to ………………………………. the minutes; overflow.
- 4 minutes; extend beyond.
- 6 minutes; remain on.
- 8 minutes; reach out.
19. If blood smears for the differential leukocyte count cannot be stained immediately, they should be dried and then fixed in for
- EDTA; 10.
- Aniline dye; 15.
- Methyl alcohol; 30.
- Buffer solution; 40.
20. When Smears for a differential leukocyte count contain a low concentration of white blood cells, but marked leukopenia, they can be prepared from the……………….layer by slowly centrifuging the blood specimen in a………………………………. tube.
- Top; volumetric.
- Buffy coat; Wintrobe hematocrit.
- Plasma layer; test.
- Red blood cell layer; Vacutainer.
21. If areas of a blood smear are still wet when staining is to begin, they will:
- Wash away.
- Stain well.
- Stain too heavily.
22. Increasing the proportion of disodium phosphate in the buffer for Wright’s stain will make the buffer more:
23. What is indicated if when staining the slide the RBCs are bluish or green?
- The stain is too acidic.
- The WOC stains very lightly.
- An alkaline stain is observed.
- The film is too thick.
24. Why should slides be stained quickly after preparation?
- So buffers will appear unequal.
- WBC distort and disintegrate quickly.
- Lines and ridges will appear.
- Acid fumes will develop.
25. Which errors should be avoided when staining slides?
- Routinely transferring of blood to the slide.
- Using an unpolished slide.
- Using clean, dust free, and smooth slides.
- Using thin films of blood and placing on slides.