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Strerilisation Cultrure Media And Pure Culture Techniques-Part 3
See all quizzes of Strerilisation Cultrure Media And Pure Culture Techniques-Part 3 here:
1 To transfer cultures from one place to
another, the device used is
a. Slant b. Needle
c. Inoculation loop d. Autoclave
2. The bacterial culture prepared by pure
culture is
a. Inoculum b. Suspension
c. Dilution d. None of these
3. Separation of a single colony is
a. Pure-culturing b. Isolation
c. Separation d. Both a and b
4. Growth period of the culture is
a. Inoculation b. Incubation
c. Incineration d. Isolation
5. At the temperature 160oC for one hour,
complete sterilization occurs in
a. Autoclave b. Hot air oven
c. Laminar flow d. Incubator
6. In autoclave, the principle involved is
a. Dry heat
b. Moist heat
c. Steam under pressur
d. Both b and c
7. The spores of th bacteria which can
withstand the moist heat effect also
a. Bacillus subtilis
b. Coxiella burnetti
c. Bacillus stearothermophilus
d. Pseudomonas
8. Factors on which disinfectivity of a
disinfectant depends
a. Concentration of the substance
b. Time of action
c. pH of the medium and temperature suitable
for the chemical
d. All of the above
9. Aldehydes, which are most powerful
disinfectants
a. Formaldehyde b. Acetaldehyde
c. Glutamal aldehyde d. Both a and c
10. Accridine dyes are more effective against
a. Gram positive b. Gram negative
c. Mycoplasmas d. Rickttsiae
11. The sterilizing agent is
a. Ethelene oxide b. Oxygen
c. Nitrogen d. Carbon tetrachloride
12. Salts of heavy metals used as disinfectants
are
a. Thiomersal b. Phenyl mercury
nitrate
c. Mercurochrome d. All of these
13. Cultures are prepared by penetrating the
inoculation loop with suspension into the
medium, they are
a. Stock cultures b. Stabcultures
c. Sub-cultures d. None of these
14. The principle involved in the streak plate
method is
a. Separation b. Streaking
c. Isolation d. Dilution
15. Culture media for fungi are
a. Potato dextrose agar (PDA)
b. Sabouraud’s agar
c. Czapekdox agar
d. All of the above
16. Spores of actinomycetes are very
sensitive, killed at room temperature of
a. 52oC for 30 min. b. 65oC for 30 min.
c. 70oC for 30 min. d. 43oC for 30 min.
17. The term that is used for the bacteria
which can withstand pasteurization but
does not grow at higher temperatures
a. Thermophiles
b. Extreme thermophiles
c. Thermoduric
d. Facultative thermophiles
18. A common laboratory method of
cultivating anaerobic micro-organisms is
a. Gas pack system
b. Brewer jar system
c. Pyrogallic acid over the cotton
d. None of these
19. Alkaliphiles grow at pH value between
a. 1 to 6 b. 6 to 9
c. 1 to 11 d. 7 to 12
20. The micro-organisms grow at high
salinity are
a. Osmophiles b. Halophiles
c. Both a and b d. None of these
21. Non-lactose fermenting colonies seen on
Mac Conkey’s medium are
a. Salmonella typhi
b. Escherichia coli
c. Klebsiella pneumoniae
d. Shigella shigae
22. Wilson and Blair medium is used for
isolation of
a. Staphylococci b. Salmonella typhosa
c. Vibrio cholerae d. Shigella shigae
23. Laboratory diagnosis of enteric fever is
based on
a. Blood culture
b. Urine and stool culture
c. Widal test
d. All of the above
24. Shigella was first isolated by
a. Shiga b. Schmitz
c. Sonnei d. Robert Koch
25. Which of the following are gas producing
Salmonella?
a. S.typhi b. S.enteritidis
c. S.cholerasuis d. S.typhimurium
